RNA Folding: Methods and Protocols

RNA Folding: Methods and Protocols

RNA Folding: Methods and Protocols, edited by Ronny Lorenz, explores computational and experimental approaches for understanding, predicting, and analyzing RNA structures. Part of the Methods in Molecular Biology series, it covers RNA folding energy models, dynamic programming algorithms, conserved structures, RNA sequence design, and folding kinetics. Featuring step-by-step protocols, practical guidance, and troubleshooting tips, the book supports researchers investigating RNA structure, molecular function, and computational biology.

Key Features:

• Introduces the principles of RNA folding and structural prediction
• Explains nearest-neighbor energy models and RNA folding energy parameters
• Covers dynamic programming algorithms for analyzing possible RNA structures
• Explores sequence evolution and conserved structures across multiple RNA sequences
• Discusses computational approaches for RNA sequence and structure design
• Examines positive and negative design objectives in RNA engineering
• Introduces kinetic models for understanding RNA folding over time
• Provides step-by-step, reproducible laboratory protocols
• Lists the materials, reagents, and procedures required for experimental methods
• Includes troubleshooting advice and practical guidance for RNA research