Lesions and Transplantation

Methods in Neurosciences, Volume 7: Lesions and Transplantation is a focused, in-depth resource that provides neuroscientists and experimental researchers with comprehensive methodologies for inducing lesions and evaluating transplants in the nervous system. This volume is a part of the well-regarded Methods in Neurosciences series, known for delivering practical laboratory protocols and experimental strategies in neurobiology.

This edition brings together a wide array of lesioning techniques—both chemical and mechanical—paired with detailed approaches for neural transplantation, implant preparation, and graft integration. Covering a diverse range of model systems and anatomical targets, the book offers guidance on how to design, implement, and assess lesion and transplantation models to investigate neural development, plasticity, injury, and repair.

Drawing from contributions by leading investigators in the field, this volume is an essential reference for any researcher involved in neurodegeneration, spinal cord injury, brain repair, or regenerative medicine.

Key Features:

• Comprehensive Methodologies: Detailed protocols for chemical lesioning (e.g., excitotoxins, acromelic acid) and nonchemical techniques, including mechanical and ischemic models like endothelin-1-induced brain lesions.

• Model Systems Coverage: Includes both traditional and emerging animal models used to study neural injury and repair, including spinal cord and hippocampal systems.

• Innovative Transplant Techniques: Practical information on preparation and integration of neural implants and fetal grafts, including novel approaches such as in oculo transplants.

• Functional Assessment Strategies: Emphasis on post-lesion and post-transplant evaluation, covering neurochemical assays, behavioral testing, and electrophysiological analysis.

• Interdisciplinary Utility: Designed for neuroscientists, but also highly valuable to biomedical researchers working in neuroregeneration, pharmacology, and experimental therapeutics.

• Rich Experimental Detail: Includes troubleshooting tips, reagent preparation, and considerations for experimental reproducibility and interpretation.

• Historical and Current Context: Highlights the evolution of lesion and transplant models in neuroscience, establishing a solid foundation for modern regenerative research.