Freshney's Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

Animal Cell Culture

Freshney’s Culture of Animal Cells is a comprehensive laboratory manual covering the principles, techniques, equipment, and applications of animal cell and tissue culture. The 8th edition includes updated protocols and emerging technologies such as CRISPR/Cas9, induced pluripotent stem cells, 3D culture, organ-on-chip, live-cell imaging, microfluidics, and high-throughput screening. It also covers aseptic technique, media preparation, primary cultures, cell-line authentication, cryopreservation, safety, reproducibility, and laboratory troubleshooting.

Key Features

• Animal cell culture
• Tissue culture techniques
• Laboratory protocols
• Aseptic technique
• Culture media and substrates
• Primary cell culture
• Cell lines and authentication
• Cryopreservation
• Mycoplasma testing
• Stem cell culture
• iPSC generation and culture
• CRISPR/Cas9 gene editing
• 3D cell culture
• Live-cell imaging
• Organ-on-chip technology
• Microfluidics and automation
• High-throughput screening
• Toxicity testing
• Reproducibility and quality control