Fluorescence Imaging of the Brain

Master Fluorescence Imaging of the Brain: Cutting-Edge Protocols for Cellular and Network-Level Insight

Step into the forefront of neuroimaging with this meticulously detailed volume offering advanced fluorescence-based protocols for exploring brain function across scales. Expert-guided and richly illustrated, this text offers both conceptual clarity and practical precision for researchers at any level.

Key Features:

Super‑Resolution and Nanoscopic Detail: Covers state-of-the-art optical methods for imaging molecules and subcellular compartments, enabling researchers to visualize neural microarchitecture and dynamic nanostructure.
Synaptic, Cellular, and Circuit Imaging: Protocols span monitoring individual synapse activity, astrocyte signaling, and targeted neuronal ensembles using advanced fluorescent sensors.
Physiology-Informed Methodology: Includes in vivo neural network recordings, neurotransmitter tracking, calcium signaling imaging, and intercellular communication across neuron–astrocyte systems.
Rigorous Protocol Design: Each chapter follows Neuromethods standards with step-by-step methodology, expert “key notes,” troubleshooting advice, and clear implementation recommendations for reproducibility.
Diverse Experimental Contexts: Features CRISPR/Cas9 fluorescent albumin knock-in models for vascular imaging, synaptic photodamage assays tracking microglia–synapse interactions, and multiplexed glutamate and Ca²⁺ dynamics monitoring with cutting-edge sensors.

Ideal for neuroscientists, imaging specialists, neurophysiologists, and translational researchers, this volume provides the depth and clarity needed to deploy advanced fluorescence techniques in studies of cellular networks, brain circuits, and neurovascular dynamics.