Epitope Mapping Protocols (Methods in Molecular Biology, 1785) Third Edition 2018

Master Epitope Mapping Protocols with the Third Edition

This book is a valuable and cutting-edge resource that expands on previous editions with more detailed research on the characterization of antibody-antigen interactions. This comprehensive guide is divided into four parts, covering traditional structural biology techniques, the use of protein fragments from antigens, surface plasmon resonance spectroscopy, and methods for identifying new antigens and assessing antibody cross-reactivity.

Key Features

• Traditional Structural Biology: Part One covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography for the entire native antigen.
• Antigen Fragments: Part Two discusses binding regions within antigen sequences using methods like bacterial surface display and ELISA.
• Spectroscopic Methods: Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry for detailed analysis.
• New Antigen Identification: Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity.
• Step-by-Step Protocols: Written in the highly successful Methods in Molecular Biology series format, chapters include introductions, necessary materials, step-by-step laboratory protocols, and troubleshooting tips.