Confocal Microscopy for Biologists

Confocal Microscopy for Biologists

Confocal Microscopy for Biologists by Alan R. Hibbs is a practical guide to understanding and using confocal microscopy in biological and biomedical research. It introduces the fundamentals of light microscopy, fluorescence, digital imaging, and confocal microscopy, helping readers obtain reliable images from fixed or living cells and tissue samples. The book also explains important technical considerations, including fluorescent dyes, pinhole settings, and fluorescence emission spectra, making it a useful reference for beginners and experienced microscopy users.

Key Features

• Introduces the fundamental principles of light microscopy.
• Explains the basics of fluorescence microscopy and fluorescent dyes.
• Describes how confocal microscopy works and its research applications.
• Covers digital imaging techniques for biological samples.
• Explains imaging of fixed and living cells and tissues.
• Provides practical guidance for obtaining high-quality microscopy images.
• Discusses pinhole size and its influence on image quality.
• Includes information about fluorescence emission spectra and dyes such as Alexa 568.
• Helps readers understand and evaluate microscopy images and presented data.
• Features a detailed index for quick access to specific topics.
• Includes an extensive glossary of microscopy terminology.
• Serves as a reference for students, researchers, and advanced microscopy users.